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To determine the influence of polymethylmethacrylate (PMMA) wear particles on macrophage-osteoclast differentiation, PMMA particles were added to mouse monocytes which were cocultured with UMR 106 osteoblast-like cells in the presence of 1,25 dihydroxy vitamin D3[1,25(OH)2D3] for up to 7 days on glass coverslips and for up to 14 days on human cortical bone slices. An increase in osteoclast differentiation, as evidenced by the expression of the osteoclast-associated enzyme tartrate-resistant acid phosphatase (TRAP) and the extent of lacunar bone resorption, was observed in monocyte cultures to which PMMA had been added. Interleukin 4 (IL-4) and Leukemia Inhibitory Factor (LIF) added to these cocultures caused considerably less expression of TRAP and significant inhibition of lacunar bone resorption. This inhibitory effect was reversed by the addition of specific neutralizing antibodies to LIF and IL-4. These findings show that PMMA-wear particle-associated macrophages exhibit an enhanced capacity for differentiation to osteoclastic bone-resorbing cells.

Type

Journal article

Journal

Acta Orthop Scand

Publication Date

12/1996

Volume

67

Pages

593 - 598

Keywords

Acid Phosphatase, Animals, Biomarkers, Tumor, Bone Resorption, Cell Differentiation, Cells, Cultured, Corrosion, Growth Inhibitors, Humans, Interleukin-4, Interleukin-6, Isoenzymes, Leukemia Inhibitory Factor, Lymphokines, Macrophages, Methylmethacrylates, Mice, Osteoclasts, Tartrate-Resistant Acid Phosphatase